The Genescence ViAlive Dual Stain Kit is a fluorescence-based assay designed to evaluate cell viability by distinguishing live and dead cells. This kit utilizes fluorescein diacetate (FDA) and propidium iodide (PI) to label viable and non-viable cells, respectively. FDA stains live cells with green fluorescence as it is converted by intracellular esterases, while PI stains dead cells by binding to DNA and emitting red fluorescence.